Ten people have been arrested and 80 charges have been laid as part of an eight-month long joint forces operation led by Peel Regional Police.
13 other agencies, including Waterloo Regional Police were also part of the investigation.
Azadali Damani, 63, from Kitchener, is facing several charges including importation of heroin, and conspiracy to commit an indictable offence.
The other nine facing charges are from Woodstock and Brampton.
Police say eight simultaneous search warrants were executed across the province last Thursday.
Officers seized $140,000 worth of heroin, $65,000 worth of opium, as well as methamphetamine and marijuana.
They also found $4.5 million of stolen tractor trailer cargo and seized $50,000 in Canadian currency.
Peel Regional Police say the investigation exposed criminal connections to the United States and as far reaching as Pakistan.
"These arrests exemplify the excellent work our officers are doing to take drugs off the street and to put criminals behind bars. I want to thank Peel Regional Police investigators and all the member agencies for their involvement in this project. Criminal operations today are borderless, and the partnerships we have are vital to our success," said Peel Regional Police Chief Jennifer Evans.